The purity of the blood cell fractions ranged from 92 to 98%, and the viability fell in the range 97-99%. The throughput of diluted blood is 162 mL/h and that of whole blood is about 2 mL/h or approximately 10(10) cells/h guidelines are given for significantly increasing throughput. In the first step, a stream of diluted blood is separated into one fraction consisting of platelets and plasma and another containing blood cells. These five steps are carried out in sequence using a single apparatus, with conditions varying from step to step in accordance with theoretical guidelines in order to achieve the desired cut points. Production of the six fractions requires five steps, each yielding two fractions. Split Flow Thin Cell Fractionation (SPLITT) is good for sample pre-treatment and clean-up as well as for production purposes.Centrifugal SPLITT fractionation, a technique designed for the continuous high-resolution separation of colloids and low-density particles, is applied here to fresh human blood, producing six purified fractions consisting of proteins and lipoproteins, platelets, red blood cells, lymphocytes, monocytes, and neutrophils. The system has two outlets (one for the smaller and one for the bigger sized particles) where the separated size fractions can be constantly collected. That means a feed flow of non-separated sample can be continuously pumped into the Split Flow Thin Cell Fractionation (SPLITT). The FFF techniques separate particles in the direction of flow, separating them according to their differing elution velocities, while the SPLITT techniques. In contrast to the other FFF techniques, Split Flow Thin Cell Fractionation (SPLITT) can separate particles in the size range of 1-100 µm on a continuous basis. SPLITT is performed by pumping the sample containing liquid into the top inlet at the start of the channel, whilst simultaneously pumping a carrier. Hanno Kinkel, University of Kiel, Germany). Split flow thin-cell fractionation Split flow thin-cell fractionation (SPLITT) is a special preparative FFF technique, using gravity for separation of m-sized particles on a continuous basis. The SEM picture shows Coccolithes, which can be fractionated by using Split Flow Thin Cell Fractionation (SPLITT) (courtesy provided by Dr. Split Flow Thin Cell Fractionation (SPLITT) has applications in the biomedical field (cell separations), in the environmental area (diatoms, sediments, algae, soil particles) and in the chemical sector (polymer and silica particles). Split Flow Thin Cell (Splitt) Applications Because earth gravity is used as separation force, Split Flow Thin Cell Fractionation (SPLITT) is limited to particles down to ca. By controlling the flow rate ratios of the two inlet streams and the flow rates of the two outlet streams, the separation can be controlled and the sample can be separated in two distinct size fractions. There are different ways of performing SPLITT in the most common way, the sample containing liquid is pumped into the top inlet a’ at the beginning of the channel and a carrier liquid is at the same time pumped into the bottom inlet b’. Centrifugal SPLITT fractionation combined laminar flow hydrodynamics and centrifugal sedimentation to obtain a con- tinuous binary separation of suspended. Split Flow Thin Cell Fractionation (SPLITT) was invented and first published by Prof. Split Flow Thin Cell Fractionation (SPLITT) is a special preparative Field-Flow Fractionation technique, using earth gravity for separation of µm-sized particles on a continuous basis.
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This would mean the 'cosmic darkness' idiom was also being used for significant events - events perceived as God's judgment - that were not perceived as literally world-ending.įor example, one particularly poignant use of the 'cosmic darkness' used it for the destruction of Jerusalem's (first) temple: 2 The language of 'cosmic darkness' was carried along with the 'Day of YHWH' concept from its earliest recorded stage. If Amos was the earliest prophet to use the Day of YHWH, he was also the earliest to use the cosmic darkness in association with it. In most of the texts from the list I provided above, the 'cosmic darkness' is used in association with 'the Day of YHWH'. The 'Day of YHWH' label was used for events that would leave this or that nation undone, while the rest of the world marched on without them. An explicit example is found in Jeremiah 46, where we read about Nebuchadnezzar conquering Necho II, with this event called 'the Day of YHWH of Hosts' (verse 46.10). We find the Day of YHWH idea used over and over throughout the biblical prophets, but there is little indication they thought history would grind to a halt on that 'Day'. However, based on how he speaks about this 'day', it is generally agreed the ideas Amos utilized were around for quite a while before he came along. The 'Day of YHWH' (or 'Day of the LORD') is found earliest in the book of Amos. Obviously, a necessary question should be, How did they understand the imagery they were using? A minor detour is required to answer that. We find this picture of 'cosmic darkness' scattered throughout a variety of contexts, across several centuries-worth of prophetic material. The heavens being shaken, or collapsing.The stars being extinguished, or falling to the earth. The moon being extinguished, or turning red.The sun being extingiushed, or disappearing mid-day.The 'cosmic darkness' had a wide range of depictions, from something as typical as a thunderstorm, to something as catastrophic as a full-blown decreation of the universe. Here are several examples of this distressful picture:Īnd for good measure, a few examples from the Psalms: One such hyperbolic metaphor was 'cosmic darkness'. But they also used hyperbolic, exaggerated pictures to stress the gravity of the events they had in mind. The prophets delivered their prophecies in various ways, but they were often rich in figures of speech, using (of course) common images from their culture to help their audiences understand what it was they were saying - such as street-corner prostitutes (Ezekiel 16), economically prosperous vineyards (Isaiah 5), or a devastating plague of locusts (Joel 1-2). Their primary interest was in events that had national or cultural importance for Israel and/or Judah. 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